Ii genetic analysis of soybean mosaic virus resistance in soybean by irfan gunduz glenn r. Soybean mosaic virus smv and alfalfa mosaic virus amv are virus diseases of soybean that are transmitted by aphids as they feed on plant sap. Smv is much more studied than bean pod mottle virus. An updated analysis by obtaining 18 new genomic sequences of chinese strainsisolates. Soybean mosaic virus smv is a member of the plant virus genus potyvirus family potyviridae.
Molecular variability and genetic structure of the. Soybean glycine max is one of the legumes, susceptible to yellow mosaic disease caused by mungbean yellow mosaic india virus mymiv and mungbean yellow mosaic virus mymv infection. Genetic analysis of resistance to soybean mosaic virus in. Plant immunity against viruses view all 14 articles. Mapping and functional analysis of candidate genes involved in resistance to soybean glycine max mosaic virus strain sc3 yuan yuan national center for soybean improvement, national key laboratory for crop genetics and germplasm enhancement, key laboratory of biology and genetic improvement of soybean, ministry of agriculture, nanjing. Soybean mosaic is caused by the potyvirus soybean mosaic virus. The presence of begomoviruses was evaluated by total dna extraction dellaporta et al. To search for recombination in soybean mosaic virus smv, the causal agent of a worldwide seedborne, aphidtransmitted viral soybean disease, we obtained all fulllength genome sequences of smv as well as partial sequences encoding the nterminal most p1 protease and the cterminal most capsid protein. Assessing its incidence in plant rna viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. Rna recombination is one of the two major factors that create rna genome variability. During the virus infection, host proteins are essential in the virus. Recombination detection program rdp rdp applies a number of recombination detection and analysis methods. The genetic variation and population structure of soybean mosaic virus smv in iran was analysed through the characterization of a set of isolates collected in the soybean growing provinces of iran.
Application to watermelon mosaic virus 2 and soybean mosaic virus n. Soybean mosaic virus smv is one of 39 viruses in the bean common mosaic virus. Genetic analysis and identification of two soybean mosaic. Little is known about the genetic structure and evolutionary biology of the smv population in southwestern china.
Analyses of genetic diversity showed that different genomic regions of smv are under different evolutionary constraints and that there was no significant genetic differentiation. Soybean mosaic virus smv is a member of the genus potyvirus, the family potyviridae. Watermelon mosaic virus wmv also known as marrow mosaic virus raychaudhuri and varma, 1975. Comparison of the predicted amino acid sequence encoded by the psmv34 cdna insert to other potyvirus protein sequences revealed extensive homology with the region of the genome encoding the. Recombination analysis of soybean mosaic virus soybean mosaic virus smv, the causal agent of a worldwide seedborne, aphidtransmitted viral soybean disease, we obtained all fulllength genome sequences of smv as well as partial sequences encoding the nterminal most p1 protease and the cterminal most capsid protein. Like all potyviral genomes, the smv genome is a singlestranded, positive. Multiple studies have proved that a single dominant gene can confer resistance to several smv strains. Recombination analyses and phylogenetic reconstruction. Recombinant dna molecules containing cdna to a soybean mosaic virus smv rna genome were constructed and partial nucleotide sequences determined for two cdna inserts, psmv34 and psmv35. Molecular genetic analyses of the soybean mosaic virus nia.
In this study, 29 smv isolates were obtained from sichuan province, and the genomic regions encoding the first protein p1 and coat protein cp were sequenced. Further recombina tion analysis showed that most part of the genome was derived from bymv isolates from the phylogenic group of iv and i to emerge as a new variant of bymv infecting gladiolus. Comoviridae, is widespread in the major soybean growing areas in the united states. Genetic analysis and mapping of genes for resistance to. Molecular variability and genetic structure of the population of. Recombination analysis of soybean mosaic virus sequences. General symptoms of smv and amv infection are plant stunting, leaf distortion, rugosity and mottling, reduced pod numbers and seed discoloration. Pdf recombination analysis of soybean mosaic virus. Soybean mosaic virus smv is one of the most prevalent and important pathogens of soybean, which produces 11 proteins, and the third protein, p3, was suggested to be involved in virus movement and replication, as well as host infection.
Molecular characterization and evolutionary analysis of. Recombination within a nucleotidebindingsiteleucine. Read genetic analysis and mapping of genes for resistance to multiple strains of soybean mosaic virus in a single resistant soybean accession pi 96983, tag theoretical and applied genetics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Assessing its incidence in plant rna viruses helps. Pdf recombination analysis of soybean mosaic virus sequences reveals evidence of rna recombination between distinct pathotypes mohan babu academia. In this study, we employed transcriptomics and proteomics to study the. Additional confirmation was obtained by sequencing the recombinant clone, which showed 100% alignment with the sequence from the soybean genome, to. Cd206 using 0 1 m phosphate buffer, ph 7 2, with 0 1% sodium sulphite. Soybean mosaic virus smv, a member of the potyviridae family, is the most common virus negatively affecting yield and seed quality in soybean glycine max l.
Pi96983 rsv1 and lee68 rsv1 with distinct recombination events within the rsv1 locus. Komuro, 1962, and until recently watermelon mosaic virus type 2 wmv2, is a plant pathogenic virus that causes viral infection sometimes referred to as watermelon mosaic disease in many different plants. The complete genomes of 30 soybean mosaic virus smv isolates and strains were sequenced in this study. Buss, chairman crop and soil environmental sciences abstract this research was conducted to analyze the genetics of soybean mosaic virus smv resistance in soybean glycine max l.
Smv can also contribute to crop loss by causing seed coat mottling thus decreasing seed quality kennedy and cooper, 1967. The presence of soybean mosaic virus smv illinois severe isolate in cotyledons, and embryos from immature seeds of both midwest and merit. Gain of virulence analysis on soybean genotypes containing rsv4 genes. We used a combination of fine mapping and transcriptome sequencing to discover the candidate genes for smv resistance in qihuang1. Intra and interspecific recombination was common in china. Merr kai li national center for soybean improvement, key laboratory of biology and genetic improvement of soybean, ministry of agriculture, nanjing agricultural university, weigang 1, nanjing, 210095 china.
Citeseerx document details isaac councill, lee giles, pradeep teregowda. Recombination analysis of soybean mosaic virus sequences reveals evidence of rna recombination between distinct pathotypes. Various rsv1 alleles condition different responses to the seven g1g7 described strains of smv, including extreme resistance, localized and systemic necrosis, and mosaic symptoms. Seven smv strains, g1 through g7, and three independent smv resistance genes r genes, rsv1, rsv3.
Genetic diversity and recombination analysis based on capsid protein gene of chilli veinal mottle virus isolates from pakistan. Soybean mosaic virus smv is one of the most broadly distributed soybean glycine max l. The partial nucleotide sequence of these isolates showed a single, undifferentiated population with low genetic diversity, highly differentiated from other smv world populations. Further molecular phylogenetic analysis of the coat protein cp genes of these smv isolates from p. An updated analysis by obtaining 18 new genomic sequences of chinese strainsisolates author links open overlay panel guangcan zhou a 1 zhuqing shao a 1 fangfang ma a ping wu a xiaoyi wu a zhongyun xie a deyue yu b hao cheng b zhihua liu c zhenfeng jiang d qingshan chen d bin wang a jianqun chen a. Comparison of transcriptome differences in soybean. Genetic diversity and recombination analysis based on. Multiple genes confer resistance to soybean mosaic virus. Analysis of soybean mosaic virus genetic diversity in iran allows the. Potyvirus has previously been described as a singlelocus multiallelic gene mapping to molecular linkage group mlg f.
To rule out the occurrence of mixed infections, the presence of begomoviruses and of soybean mosaic virus smv, genus potyvirus was checked. It is one of the most devastating viral pathogens of soybean crops causing severe symptoms such as mosaic, mottling, chlorosis and rugosity in leaves, as well as reductions in plant growth with yield losses of up to 100% 14. Potyviruses constitute the largest genus of plant viruses and include many. Comprehensive analysis of soybean mosaic virus p3 protein. Phylogenetic and recombination analysis of tobacco bushy. Bean yellow mosaic virus, etic analysis, rtpcr and recombination. Analysis of soybean mosaic virus genetic diversity in iran.
It runs well under windows 9598ntxpvista78 and maymay. Recombination analysis of soybean mosaic virus sequences reveals evidence of rna recombination between distinct pathotypes, virology journal, 2008, pp. To search for recombination in soybean mosaic virus smv, the causal agent of a worldwide seedborne, aphidtransmitted viral soybean disease, we. The biological and molecular characterization of six isolates of a new cowpea mild mottle virus strain cpmmv.
Recombination analysis of soybean mosaic virus sequences reveals evidence of rna recombination between distinct pathotypes alla g gagarinova, 1, 2, 4 mohan babu, 1 martina v stromvik, 3 and aiming wang 1, 2. Identification of candidate genes for resistance to. It infects mainly plants belonging to the family fabaceae but has also been found infecting other economically important crops. Soybean mosaic virus smv is one of the most devastating pathogens that cost. Pdf analysis of soybean mosaic virus genetic diversity in iran. Mapping and functional analysis of candidate genes. The current status of the soybeansoybean mosaic virus. Characterization of recombinant bean yellow mosaic virus. Merr kai li national center for soybean improvement, key laboratory of biology and genetic improvement of soybean, ministry of agriculture, nanjing agricultural university, weigang 1.
Recombination genetic structure phylogenetic analysis genetic variation the complete genomes of 30 soybean mosaic virus smv isolates and strains were sequenced in this study. Thus, in the current study, both intensity and quality red. Shading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus smv is a biotic factor that limits the yield and quality of soybean. This study is a continuation of our earlier reports on the identification and mapping of the soybean mosaic virus smv strain sc3 resistance gene present on chromosome lg f of soybean glycine max l. Genetic evolutionary analysis of soybean mosaic virus.
Smv is the cause of soybean mosaic disease that occurs in all the soybean productions areas of the world. Molecular and biological characterization of cowpea mild. Together with fourteen previously reported sequences, we analyzed the genetic structure of the smv population. The soybean rsv1 gene for resistance to soybean mosaic virus smv. However, the reaction of j05 to smv strains in the united states of america is unknown, and genetic information is needed to utilize this germplasm in a. In nebraska, smv is not as common as bpmv based on field surveys conducted from the 2000 to 2002 growing seasons, as only 3. Also, recombinant soybean mosaic virus smvr was recently identified and classified as a novel strain in chongqing, china, exhibiting different pathogenicity on.
Comparison of the predicted amino acid sequence encoded by the psmv34 cdna insert to other potyvirus protein. Transcriptomic and proteomic analysis of yellow mosaic. The complete nucleotide sequences of the genomic rnas of the naturally occurring partial diploid strain ilcb1 were determined. Plant introduction pi 96983 has been reported to contain smv resistance genes e. Soybean cultivar j05 was identified to be resistant to the most virulent strain of soybean mosaic virus smv in northeastern china. Genetic analysis of soybean mosaic virus smv resistance genes. However, little is known about the defense response of soybean to smv in the shade. Host gene expression profiling has mostly been performed in plants infected with begomovirus under controlled conditions. Agronomic impact our experience over the past few years. For singlestranded rnaviruses, recombination is a major evolutionary event allowing isolates to adapt to new environmental conditions and hosts, and frequent recombination events have been detected for various rna viruses such as soybean mosaic virus and potyvirusisolates1416.
Characteristics of soybean mosaic virus smv soybean pathosystem smv is a disease that occurs worldwide wherever soybean is grown, causing severe yield reductions demski and kuhn, 1989. Soybean mosaic virus and alfalfa mosaic virus badger. This analysis identified many unique recombination signals. Infection of soybean seed parts and seed transmission. Soybean mosaic virus smv is seed and aphid transmitted and can. Recombination sites reported in previous studies gagarinova et al.
550 367 173 115 1321 458 1298 459 727 1151 1294 58 206 263 1361 576 1107 21 1028 158 1350 1169 685 1173 876 207 1450 424 1278 302 1127 904 713 734 1105 40 944 737 435 292 688